v1.2.1 - Bouncy Basenji Patch [2024-11-28]

Added

• #545 Updated to nf-core pipeline template v3.0.2 (added by @LilyAnderssonLee & @jfy133)

Fixed

• #550 Fixed a bug where single-end data with the same sample names that include . in it would overwrite output files (❤️ to @SannaAb for reporting, fixed by @Midnighter)
• #552 Fixed the issue where nonpareil fails due to insufficient data for calculation caused by a missing PNG (❤️ to @Joon-Klaps for reporting, fixed by @LilyAnderssonLee)

Dependencies

Deprecated

v1.2.0 - Bouncy Basenji [2024-10-03]

Added

• #417 Added reference-free metagenome complexity/coverage estimation with Nonpareil (added by @jfy133)
• #466 Input database sheets can specify a db_type column to distinguish between short- and long-read databases (added by @LilyAnderssonLee)
• #505 Add small files to the file tower.yml (added by @LilyAnderssonLee)
• #508 Add nanoq as a filtering tool for nanopore reads (added by @LilyAnderssonLee)
• #511 Add porechop_abi as an alternative adapter removal tool for long reads nanopore data (added by @LilyAnderssonLee)
• #512 Update all tools to the latest version and include nf-test (updated by @LilyAnderssonLee & @jfy133)
• #537 Update the module motus/merge to the latest version (Updated by @sofstam & @LilyAnderssonLee)

Fixed

• #518 Fixed a bug where Oxford Nanopore FASTA input files would not be processed (❤️ to @ikarls for reporting, fixed by @jfy133)
• #523 Removed hardcoded -m lca from GANON_CLASSIFY due to more options in new version of ganon (fixed by @LilyAnderssonLee & @jfy133)
• #531 Fix FASTA input validation in schema allowing FASTQ extension, expand allowed FASTA extensions (fixed by @jfy133)
• #512 Minor formatting and ordering improvements in MultiQC report (by @jfy133)
• #532 - Added missing documentation behind the 'ignore' BRACKEN_BRACKEN error strategy (❤️ to @Mavti for reporting, fixed by @jfy133)
• #536 - Redefine contents_re for filtlong to fix its missing from the MultiQC report (fixed by @LilyAnderssonLee)

Dependencies

Tool	Previous version	New version
bbmap	39.01	39.06
bowtie2	2.4.4	2.5.2
bracken	2.7	2.9
diamond	2.0.15	2.1.8
ganon	1.5.1	2.0.0
kraken2	2.1.2	2.1.3
krona	2.8	2.8.1
megan	6.24.20	6.25.9
metaphlan	4.0.6	4.1.1
minimap2	2.24	2.28
motus	3.0.3	3.1.0
multiqc	1.21	1.25
samtools	1.17	1.20

Deprecated

v1.1.8 - Augmented Akita Patch [2024-06-20]

Added

• #487 Updated to nf-core pipeline template v2.14.1 (added by @jfy133)

Fixed

• #484 Improved input validation to immediately fail if run accession IDs within a given sample ID are not unique (❤️ to @sofstam for reporting, fixed by @jfy133)
• #491 Added flag to publish intermediate bracken files (❤️ to @ewissel for reporting, fixed by @sofstam and @jfy133)
• #489 Fix KrakenUniq classified reads output format mismatch (❤️ to @SannaAb for reporting, fixed by @jfy133)
• #495 Stop TAXPASTA failures when profiles do not have exact compositionality (fixes by @Midnighter, @jfy133)

Dependencies

Tool	Previous version	New version
KMCP	0.9.1	0.9.4
TAXPASTA	0.6.1	0.7.0

Deprecated

• #492 Removed --kmcp_mode parameter from KMCP to allow per database specification by setting in db_params in database sheet (fixed by @jfy133)

v1.1.7 - Augmented Akita Patch [2024-04-29]

Added

• #477 Provide more emphasis and links to tutorials on how to retrieve and supply reference databases (❤️ to @vmkalbskopf for reporting, added by @jfy133)

Fixed

• #476 Fixed bug in validating Bracken/Kraken/KMCP split database parameters (fixed by @LilyAnderssonLee)

Dependencies

Deprecated

v1.1.6 - Augmented Akita Patch [2024-04-16]

Added

• #454 Updated to nf-core pipeline template v2.13.1 (added by @LilyAnderssonLee & @sofstam)
• #461 Turned on 'strict' Nextflow evaluation runs (added by @jfy133)
• #461 Optimised database compression so each compressed input database is untarred once, and shared amongst each run with different parameters (added by @jfy133)
• #461 Added new parameter to optionally save uncompressed databases (added by @jfy133)
• #471 Removed -stub run in the download_pipeline.yml because the pipeline does not support stub runs on dev (fixed by @LilyAnderssonLee)

Fixed

• #336 Replaced samplesheet check with nf-validation for both sample and database input sheets (fix by @LilyAnderssonLee)
• #460 Corrected the channel transformations to combine Kaiju and mOTUs reports with their reference databases (fix by @Midnighter)

v1.1.5 - Augmented Akita Patch [2024-02-08]

Added

• #439 Read deduplication with fastp (added by @maxibor)
• #440 Include mention of pre-built kaiju databases in tutorial.md (added by @Joon-Klaps)
• #442 Updated to nf-core pipeline template v2.12 (added by @sofstam)

Fixed

• #444 Centrifuge now uses dedicated tmp directory to hopefully prevent mkfifo clashes (❤️ to @erinyoung for reporting, fix by @jfy133)

Dependencies

Tool	Previous version	New version
Centrifuge	1.0.4_beta	1.0.4.1

v1.1.4 - Augmented Akita Patch [2024-01-24]

Fixed

• #431 Updated kaiju2table module to report taxon names (fix by @Joon-Klaps)
• #430 Fix the fastq output in the module LONGREAD_HOSTREMOVAL. (fix by @LilyAnderssonLee)

Dependencies

Tool	Previous version	New version
kaiju	1.8.2	1.10.0

v1.1.3 - Augmented Akita Patch [2024-01-12]

Added

• #424 Updated to nf-core pipeline template v2.11.1 (added by @LilyAnderssonLee & @sofstam)

Fixed

• #419 Added improved syntax highlighting for tables in documentation (fix by @mashehu)
• #421 Updated the krakenuniq/preloadedkrakenuniq module that contained a fix for saving the output reads (❤️ to @SannaAb for reporting, fix by @Midnighter)
• #427 Fixed preprint information in the recommended methods text (fix by @jfy133)

Dependencies

Tool	Previous version	New version
multiqc	1.15	1.19
fastqc	11.9	12.1
nf-validation	unpinned	1.1.3

v1.1.2 - Augmented Akita Patch [2023-10-27]

Added

• #408 Added preprint citation information to README and manifest (added by @jfy133)

Fixed

• #405 Fix database to tool mismatching in KAIJU2KRONA input (❤️ to @MajoroMask for reporting, fix by @jfy133)
• #406 Fix overwriting of bracken-derived kraken2 outputs when the database name is shared between Bracken/Kraken2. (❤️ to @MajoroMask for reporting, fix by @jfy133)
• #409 Fix a NullPointerException error occurring occasionally in older version of MEGAN's rma2info (❤️ to @MajoroMask for reporting, fix by @jfy133)

Dependencies

Tool	Previous version	New version
megan/rma2info	6.21.7	6.24.20

v1.1.1 - Augmented Akita Patch [2023-10-11]

Added

• #379 Added support for previously missing Bracken-corrected Kraken2 report as output (added by @hkaspersen & @jfy133 )
• #380 Updated to nf-core pipeline template v2.10 (added by @LilyAnderssonLee & @sofstam)
• #393 Add validation check for a taxpasta taxonomy directory if --taxpastaadd* parameters requested (♥️ to @alimalrashed for reporting, added by @jfy133)

Fixed

• #383 Update the module of KrakenUniq to the latest to account for edge case bugs where FASTQ input was mis-detected as wrong format (❤️ to @asafpr for reporting and solution, fixed by @LilyAnderssonLee)
• #392 Update the module of Taxpasta to support adding taxa information to results (❤️ to @SannaAb for reporting, fixed by @Midnighter)

Dependencies

Tool	Previous version	New version
KrakenUniq	1.0.2	1.0.4
taxpasta	0.6.0	0.6.1

Deprecated