A repository where I practice scRNA-seq analysis by re-analyzing my favorite papers.
All analysis include documentation and simple walkthroughs for anyone who would like to replicate this work.
RNA sequencing is an approach to detect and quantify RNA molecules in a biological sample. The problem with this approach is that it gives a collective report of all the RNAs analyzed in a tissue, hindering the direct assessment of the fundamental unit of biology—the cell. Single Cell RNA-seq (scRNA-seq) solves this problem by single cell isolation and indexing. This allows for RNA detection and quantification at the cell level, giving a histological insight on the sample that can have multiple applications (Haque et. al., 2017).