Support images_by_name for Screen and Plate

src/omero_metadata/populate.py

@@ -225,11 +225,6 @@ def create_columns(self):
     def columns_sanity_check(self, columns):
         column_types = [column.__class__ for column in columns]
         column_names = [column.name for column in columns]
-        if WellColumn in column_types and ImageColumn in column_types:
-            log.debug(column_types)
-            raise MetadataError(
-                ('Well Column and Image Column cannot be resolved at '
-                 'the same time. Pick one.'))
         if RoiColumn in column_types and ROI_NAME_COLUMN in column_names:
             log.debug('Found both ROI names and IDs. Not appending either.')
             return False
@@ -390,8 +385,8 @@ def get_well_name(self, well_id, plate=None):
         row = self.AS_ALPHA[row]
         return '%s%d' % (row, col + 1)
 
-    def get_image_id_by_name(self, iname, dname=None):
-        return self.wrapper.get_image_id_by_name(iname, dname)
+    def get_image_id_by_name(self, iname, did=None):
+        return self.wrapper.get_image_id_by_name(iname, did)
 
     def get_image_name_by_id(self, iid, pid=None):
         return self.wrapper.get_image_name_by_id(iid, pid)
@@ -574,7 +569,8 @@ def get_image_name_by_id(self, iid, pid=None):
             raise Exception("Cannot resolve image to plate")
         return self.images_by_id[pid][iid].name.val
 
-    def parse_plate(self, plate, wells_by_location, wells_by_id, images_by_id):
+    def parse_plate(self, plate, wells_by_location, wells_by_id,
+                    images_by_id, images_by_name):
         """
         Accepts PlateData instances
         """
@@ -594,6 +590,7 @@ def parse_plate(self, plate, wells_by_location, wells_by_id, images_by_id):
             for well_sample in well.well_samples:
                 image = well_sample.image
                 images_by_id[image.id.val] = image
+                images_by_name[image.name.val] = image
         log.debug('Completed parsing plate: %s' % plate.name.val)
         for row in wells_by_location:
             log.debug('%s: %r' % (row, list(wells_by_location[row].keys())))
@@ -638,6 +635,9 @@ def __init__(self, value_resolver):
         super(ScreenWrapper, self).__init__(value_resolver)
         self._load()
 
+    def get_image_id_by_name(self, iname, pid=None):
+        return self.images_by_name[pid][iname].id.val
+
     def get_plate_name_by_id(self, plate):
         plate = self.plates_by_id[plate]
         return plate.name.val
@@ -666,6 +666,7 @@ def _load(self):
         if self.target_object is None:
             raise MetadataError('Could not find target object!')
         self.target_name = unwrap(self.target_object.getName())
+        self.images_by_name = dict()
         self.images_by_id = dict()
         self.wells_by_location = dict()
         self.wells_by_id = dict()
@@ -689,8 +690,11 @@ def _load(self):
             wells_by_id = dict()
             self.wells_by_location[plate.name.val] = wells_by_location
             self.wells_by_id[plate.id.val] = wells_by_id
+            images_by_name = dict()
+            self.images_by_name[plate.id.val] = images_by_name
             self.parse_plate(
-                plate, wells_by_location, wells_by_id, images_by_id
+                plate, wells_by_location, wells_by_id,
+                images_by_id, images_by_name
             )
 
 
@@ -705,6 +709,10 @@ def get_well_by_id(self, well_id, plate=None):
         wells = self.wells_by_id[plate]
         return wells[well_id]
 
+    def get_image_id_by_name(self, iname, pid=None):
+        plate = self.target_object.id.val
+        return self.images_by_name[plate][iname].id.val
+
     def subselect(self, rows, names):
         """
         If we're processing a plate but the bulk-annotations file contains
@@ -742,18 +750,22 @@ def _load(self):
         self.images_by_id = dict()
         images_by_id = dict()
 
+        self.images_by_name = dict()
+        images_by_name = dict()
+        self.images_by_name[self.target_object.id.val] = images_by_name
+
         self.wells_by_location[self.target_object.name.val] = wells_by_location
         self.wells_by_id[self.target_object.id.val] = wells_by_id
         self.images_by_id[self.target_object.id.val] = images_by_id
         self.parse_plate(
             PlateData(self.target_object),
-            wells_by_location, wells_by_id, images_by_id
+            wells_by_location, wells_by_id, images_by_id, images_by_name
         )
 
 
 class PDIWrapper(ValueWrapper):
 
-    def get_image_id_by_name(self, iname, dname=None):
+    def get_image_id_by_name(self, iname, did=None):
         raise Exception("to be implemented by subclasses")
 
 
@@ -793,7 +805,7 @@ def resolve_shape(self, value):
             log.warn('Wrong input type for Shape ID: %s' % value)
             return -1
 
-    def get_image_id_by_name(self, iname, dname=None):
+    def get_image_id_by_name(self, iname, did=None):
         return self.images_by_name[iname].id.val
 
     def get_image_name_by_id(self, iid, did):
@@ -887,8 +899,8 @@ def __init__(self, value_resolver):
         self.datasets_by_name = dict()
         self._load()
 
-    def get_image_id_by_name(self, iname, dname=None):
-        return self.images_by_name[dname][iname].id.val
+    def get_image_id_by_name(self, iname, did=None):
+        return self.images_by_name[did][iname].id.val
 
     def get_image_name_by_id(self, iid, did=None):
         return self.images_by_id[did][iid].name.val
@@ -1218,11 +1230,6 @@ def parse(self):
                     return self.parse_from_handle_stream(f2)
 
     def preprocess_data(self, reader):
-        # Get count of data columns - e.g. NOT Well Name
-        column_count = 0
-        for column in self.columns:
-            if column.name not in ADDED_COLUMN_NAMES:
-                column_count += 1
         for i, row in enumerate(reader):
             row = [(self.columns[i], value) for i, value in enumerate(row)]
             for column, original_value in row:
@@ -1454,6 +1461,7 @@ def post_process(self):
                     DatasetI is target_class or
                     ProjectI is target_class) and \
                     resolve_image_ids and not resolve_image_names:
+                # PDI - need to know Image IDs from Names
                 iid = -1
                 try:
                     log.debug(image_column)
@@ -1471,12 +1479,14 @@ def post_process(self):
                         iname, did)
                 except KeyError:
                     log.warn(
-                        "%d not found in image ids" % iid)
+                        "%s not found in image names" % iname)
                 assert i == len(image_column.values)
                 image_column.values.append(iid)
             elif image_name_column is not None and (
                     ScreenI is target_class or
-                    PlateI is target_class):
+                    PlateI is target_class) and \
+                    resolve_image_names and not resolve_image_ids:
+                # HCS - we need to know image Names from IDs
                 iid = image_column.values[i]
                 log.info("Checking image %s", iid)
                 pid = None
@@ -1487,6 +1497,27 @@ def post_process(self):
                 image_name_column.size = max(
                     image_name_column.size, len(iname)
                 )
+            elif image_name_column is not None and (
+                    ScreenI is target_class or
+                    PlateI is target_class) and \
+                    resolve_image_ids and not resolve_image_names:
+                # HCS - we need to know image IDs from Names
+                log.debug(image_column)
+                iname = image_name_column.values[i]
+                iid = -1
+                try:
+                    # If target class is Screen, a plate column should exist
+                    if "plate" in columns_by_name:
+                        pid = int(columns_by_name["plate"].values[i])
+                    elif target_class is PlateI:
+                        pid = self.target_object.id.val
+                    log.debug("Using Plate:%d" % pid)
+                    iid = self.value_resolver.get_image_id_by_name(
+                        iname, pid)
+                except KeyError:
+                    log.warn(
+                        "%s not found in image names" % iname)
+                image_column.values.append(iid)
             elif target_class is not ImageI:
                 log.info('Missing image name column, skipping.')
 

test/integration/metadata/test_populate.py

@@ -144,12 +144,27 @@ def create_screen(self, row_count, col_count):
                                          plate_cols=col_count)[0]
         plate2 = self.test.import_plates(plate_rows=row_count,
                                          plate_cols=col_count)[0]
+        # Rename Images like "A1_Field-0" to match names in csv
+        conn = BlitzGateway(client_obj=self.test.client)
+        update = conn.getUpdateService()
+        images_by_id = {}
+        for pid in [plate1.id.val, plate2.id.val]:
+            plate = conn.getObject("Plate", pid)
+            for well in plate.listChildren():
+                for field_index, ws in enumerate(well.listChildren()):
+                    img = ws.getImage()._obj
+                    img.name = rstring(
+                        f'{ well.getWellPos() }_Field-{field_index}')
+                    img = update.saveAndReturnObject(img)
+                    images_by_id[img.id.val] = img
         plate1 = self.set_name(plate1, "P001")
         plate2 = self.set_name(plate2, "P002")
         screen = ScreenI()
         screen.name = rstring("Screen")
         screen.linkPlate(plate1.proxy())
         screen.linkPlate(plate2.proxy())
+        # cache images_by_id for checking result
+        self.images_by_id = images_by_id
         return self.test.client.sf.getUpdateService().\
             saveAndReturnObject(screen)
 
@@ -176,11 +191,18 @@ def assert_columns(self, columns):
         assert col_names == ",".join([c.name for c in columns])
 
     def assert_values(self, row_values):
+        # Check rows, based on self.create_csv()
         # Unsure where the lower-casing is happening
         if "A1" in row_values or "a1" in row_values:
             assert "Control" in row_values
         elif "A2" in row_values or "a2" in row_values:
             assert "Treatment" in row_values
+        elif "roi1" in row_values:
+            assert 0.5 in row_values
+            assert 100 in row_values
+        elif "roi2" in row_values:
+            assert 'nan' in [str(value) for value in row_values]
+            assert 200 in row_values
 
     def assert_child_annotations(self, oas):
         for ma, wid, wr, wc in oas:
@@ -208,14 +230,16 @@ def get_all_map_annotations(self):
 class Screen2Plates(Fixture):
 
     def __init__(self):
-        self.count = 6
+        self.count = 8
         self.ann_count = 4
         self.row_count = 1
         self.col_count = 2
         self.csv = self.create_csv(
-            col_names="Plate,Well,Well Type,Concentration",
-            row_data=("P001,A1,Control,0", "P001,A2,Treatment,10",
-                      "P002,A1,Control,0", "P002,A2,Treatment,10"))
+            col_names="Plate,Well,Image Name,Well Type,Concentration",
+            row_data=("P001,A1,A1_Field-0,Control,0",
+                      "P001,A2,A2_Field-0,Treatment,10",
+                      "P002,A1,A1_Field-0,Control,0",
+                      "P002,A2,A2_Field-0,Treatment,10"))
         self.screen = None
 
     def assert_row_count(self, rows):
@@ -225,10 +249,19 @@ def assert_row_count(self, rows):
         assert rows == 2 * self.row_count * self.col_count
 
     def assert_columns(self, columns):
-        # Adds Plate Name,Well Name columns
-        col_names = "Plate,Well,Well Type,Concentration,Plate Name,Well Name"
+        # Adds Plate Name,Well Name, Image columns
+        col_names = ("Plate,Well,Image Name,Well Type,"
+                     "Concentration,Plate Name,Well Name,Image")
         assert col_names == ",".join([c.name for c in columns])
 
+    def assert_values(self, row_values):
+        super(Screen2Plates, self).assert_values(row_values)
+        # last column should contain valid Image ID
+        image_id = row_values[-1]
+        image_name = row_values[2]
+        assert image_id in self.images_by_id
+        assert self.images_by_id[image_id].name.val == image_name
+
     def get_target(self):
         if not self.screen:
             self.screen = self.create_screen(self.row_count, self.col_count)