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update BRAKER (#43)
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* update BRAKER

* Update BRAKER.md

Signed-off-by: Cal <[email protected]>

---------

Signed-off-by: Cal <[email protected]>
Co-authored-by: Cal <[email protected]>
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DininduSenanayake and CallumWalley authored Dec 5, 2023
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hidden: false
position: 0
tags: []
title: BRAKER
vote_count: 0
vote_sum: 0
zendesk_article_id: 6529511928207
zendesk_section_id: 360000040076
---



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## Description

The rapidly growing number of sequenced genomes requires fully automated
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## Prerequisites


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!!! prerequisite Obtain GeneMark-ES/ET Academic License 
GeneMark-ES/ET which is one of the dependencies for BRAKER requires an
individual academic license  (this is free). This can be obtained as
below
- Download URL
<http://topaz.gatech.edu/genemark/license_download.cgi>


!!! prerequisite "Obtain GeneMark-ES/ET Academic License"

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GeneMark-ES/ET which is one of the dependencies for BRAKER requires an individual academic license  (this is free). This can be obtained as below
- Download URL <http://topaz.gatech.edu/genemark/license_download.cgi>
- ![genemark\_es\_license.png](../../assets/images/BRAKER.png)
- Downloaded filename will be in the format of **gm\_key\_64.gz. **

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- Decompress this file with `gunzip gm_key_64.gz`  and move it to
home directory as  a **hidden** file under the filename `.gm_key`
 .i.e. `~/.gm_key`
home directory as  a **hidden** file under the filename `.gm_key` .i.e. `~/.gm_key`

!!! prerequisite Copy AUGUSTUS config to a path with read/write permissions
Make a copy of AUGUSTUS config from
***/opt/nesi/CS400\_centos7\_bdw/AUGUSTUS/3.4.0-gimkl-2022a/config***
 to path with read/write permissions .i.e. project, nobackup,home 
!!! info "Copy AUGUSTUS config to a path with read/write permissions"

Make a copy of AUGUSTUS config from ***/opt/nesi/CS400\_centos7\_bdw/AUGUSTUS/3.4.0-gimkl-2022a/config*** to path with read/write permissions .i.e. project, nobackup,home 

### Example Slurm scripts

Following example uses the .fa files provided BRAKER developers on
<https://github.com/Gaius-Augustus/BRAKER/tree/master/example>
Following example uses the [.fa files provided BRAKER developers](https://github.com/Gaius-Augustus/BRAKER/tree/master/example)

``` sl
#!/bin/bash -e
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augustus.hints_iter1.codingseq evidence.gff hintsfile_iter1.gff what-to-cite.txt
augustus.hints_iter1.gff GeneMark-EP/ prevHints.gff
augustus.hints_iter1.gtf GeneMark-ES/ proteins.fa
```
```

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