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Error: spades-hammer, OS return value: 1 #1313

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osvatic opened this issue Jun 11, 2024 · 2 comments
Open
1 task done

Error: spades-hammer, OS return value: 1 #1313

osvatic opened this issue Jun 11, 2024 · 2 comments

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@osvatic
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osvatic commented Jun 11, 2024

Description of bug

Spades-hammer is terminating because of a segmentation fault while kmer-counting and reports an "OS return value: 1".

Command line:

/lisc/scratch/jmf/internal/Analyses_Jay/JMF_2108_11/.snakemake/conda/0ae2b4a3475f1b8ec4109c90dec7e0d2_/bin/spades.py      -t      48      -m      1000    -k      21,31,41,51,61,71,81,91,101,111,121     --meta  --pe-12 1       /lisc/scratch/jmf/internal/Analyses_Jay/JMF_2108_11/data/interleave_DNA/JMF-2108-11-0010C.interleave.fastq.gz   -o      /lisc/scratch/jmf/internal/Analyses_Jay/JMF_2108_11/results/JMF-2108-11-0010C_spades 

Details from spades.log:
Analyses_Jay/JMF_2108_11/results/JMF-2108-11-0010C_spades/split_input/JMF-2108-11-0010C.interleave_1.fastq
0:01:18.914 329M / 897M INFO K-mer Counting (kmer_data.cpp : 324) Processed 1201470 reads
0:01:18.915 329M / 897M INFO K-mer Counting (kmer_data.cpp : 315) Processing /lisc/scratch/jmf/internal/Analyses_Jay/JMF_2108_11/results/JMF-2108-11-0010C_spades/split_input/JMF-2108-11-0010C.interleave_2.fastq
The program was terminated by segmentation fault
=== Stack Trace ===
/lisc/scratch/jmf/internal/Analyses_Jay/JMF_2108_11/.snakemake/conda/0ae2b4a3475f1b8ec4109c90dec7e0d2_/bin/spades-hammer(+0x1b3d7) [0x55bce55dd3d7]
/lisc/scratch/jmf/internal/Analyses_Jay/JMF_2108_11/.snakemake/conda/0ae2b4a3475f1b8ec4109c90dec7e0d2_/bin/spades-hammer(+0x1bf06) [0x55bce55ddf06]
/lib64/libc.so.6(+0x3e6f0) [0x7fbbc903e6f0]
/lisc/scratch/jmf/internal/Analyses_Jay/JMF_2108_11/.snakemake/conda/0ae2b4a3475f1b8ec4109c90dec7e0d2_/bin/spades-hammer(+0x8e83f) [0x55bce565083f]
/lisc/scratch/jmf/internal/Analyses_Jay/JMF_2108_11/.snakemake/conda/0ae2b4a3475f1b8ec4109c90dec7e0d2_/bin/spades-hammer(+0x908f3) [0x55bce56528f3]
/lisc/scratch/jmf/internal/Analyses_Jay/JMF_2108_11/.snakemake/conda/0ae2b4a3475f1b8ec4109c90dec7e0d2_/bin/spades-hammer(+0x4a1f6) [0x55bce560c1f6]
/lisc/scratch/jmf/internal/Analyses_Jay/JMF_2108_11/.snakemake/conda/0ae2b4a3475f1b8ec4109c90dec7e0d2_/bin/spades-hammer(+0x4a3d6) [0x55bce560c3d6]
/lisc/scratch/jmf/internal/Analyses_Jay/JMF_2108_11/.snakemake/conda/0ae2b4a3475f1b8ec4109c90dec7e0d2_/bin/../lib/libgomp.so.1(+0x186d9) [0x7fbbc932f6d9]
/lib64/libc.so.6(+0x89c12) [0x7fbbc9089c12]
/lib64/libc.so.6(+0x10ecc0) [0x7fbbc910ecc0]

== Error == system call for: "['/lisc/scratch/jmf/internal/Analyses_Jay/JMF_2108_11/.snakemake/conda/0ae2b4a3475f1b8ec4109c90dec7e0d2_/bin/spades-hammer', '/lisc/scratch/jmf/internal/Analyses_Jay/JMF_2108_11/results/JMF-2108-11-0010C_spades/corrected/configs/config.info']" finished abnormally, OS return value: 1
None

This seems to be specific to a certain dataset as I have no problems running SPAdes on others within this project.

spades.log

spades.log

params.txt

params.txt

SPAdes version

SPAdes 3.15.5

Operating System

Linux-5.14.0-362.24.1.0.1.el9_3.x86_64-x86_64-with-glibc2.34

Python Version

version: 3.12.3

Method of SPAdes installation

unkown

No errors reported in spades.log

  • Yes
@asl
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asl commented Jun 11, 2024

Does the problem reproduce with the latest SPAdes 4.0?

@osvatic
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osvatic commented Jun 12, 2024

Yes, this is reproducible in SPAdes 4.0. The OS return value is the same and it looks like it gets stuck at the same place.

params.txt
spades.log

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