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Merge pull request #87 from BodenmillerGroup/cytoviewer_updates
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cytoviewer citation update
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lassedochreden authored Jan 5, 2024
2 parents 9ff9b7d + 16919aa commit 634dcaf
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7 changes: 4 additions & 3 deletions 06-quality_control.Rmd
Original file line number Diff line number Diff line change
Expand Up @@ -62,7 +62,7 @@ sizes.
An easier and interactive way of observing segmentation quality is to use the
interactive image viewer provided by the
[cytoviewer](https://github.com/BodenmillerGroup/cytoviewer) R/Bioconductor
package [@Meyer2023]. Under "Image-level" > "Basic controls", up to six markers
package [@Meyer2024]. Under "Image-level" > "Basic controls", up to six markers
can be selected for visualization. The contrast of each marker can be adjusted.
Under "Image-level" > "Advanced controls", click the "Show cell outlines" box
to outline segmented cells on the images.
Expand All @@ -72,11 +72,12 @@ library(cytoviewer)
app <- cytoviewer(image = images,
mask = masks,
object = spe,
cell_id = "ObjectNumber",
img_id = "sample_id")
if (interactive()) {
shiny::runApp(app, launch.browser = TRUE)
shiny::runApp(app)
}
```

Expand Down Expand Up @@ -395,7 +396,7 @@ samples or batches of samples. Observing potential staining differences can be
crucial to assess data quality. We will use ridgeline visualizations to check
differences in staining patterns:

```{r ridges, message=FALSE, fig.width=7, fig.height=25}
```{r ridges, message=FALSE, warning = FALSE, fig.width=7, fig.height=25}
multi_dittoPlot(spe, vars = rownames(spe)[rowData(spe)$use_channel],
group.by = "patient_id", plots = "ridgeplot",
assay = "exprs",
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