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Differentiation of maternal and fetal sources #224

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Gh-Genetics opened this issue Mar 17, 2024 · 0 comments
Open

Differentiation of maternal and fetal sources #224

Gh-Genetics opened this issue Mar 17, 2024 · 0 comments

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@Gh-Genetics
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Error: Unable to open file ./mutai/cow/C3-1/C3-1.QC.vcf. Exiting.
Traceback (most recent call last):
File "/opt/souporcell/souporcell_pipeline.py", line 586, in
final_vcf = freebayes(args, bam, fasta)
File "/opt/souporcell/souporcell_pipeline.py", line 398, in freebayes
subprocess.check_call(["bedtools", "intersect", "-wa", "-a", args.common_variants, "-b", args.out_dir + "/depth_merged.bed"], stdout = vcf)
File "/opt/conda/envs/py36/lib/python3.6/subprocess.py", line 311, in check_call
raise CalledProcessError(retcode, cmd)
subprocess.CalledProcessError: Command '['bedtools', 'intersect', '-wa', '-a', './mutai/cow/C3-1/C3-1.QC.vcf', '-b', './output/depth_merged.bed']' returned non-zero exit status 1.

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