diff --git a/src/EmbedIO/Net/HttpListener.cs b/src/EmbedIO/Net/HttpListener.cs
index 2f96ffb2..e095f942 100644
--- a/src/EmbedIO/Net/HttpListener.cs
+++ b/src/EmbedIO/Net/HttpListener.cs
@@ -2,6 +2,7 @@
 using System.Collections.Concurrent;
 using System.Collections.Generic;
 using System.Linq;
+using System.Net.Security;
 using System.Security.Cryptography.X509Certificates;
 using System.Threading;
 using System.Threading.Tasks;
@@ -27,15 +28,17 @@ public sealed class HttpListener : IHttpListener
         /// Initializes a new instance of the <see cref="HttpListener" /> class.
         /// </summary>
         /// <param name="certificate">The certificate.</param>
-        public HttpListener(X509Certificate? certificate = null)
+        /// <param name="clientCertificateValidationCallback">The client certificate validator</param>
+        public HttpListener(X509Certificate? certificate = null, RemoteCertificateValidationCallback? clientCertificateValidationCallback = null)
         {
             Certificate = certificate;
+            ClientCertificateValidationCallback = clientCertificateValidationCallback;
 
             _prefixes = new HttpListenerPrefixCollection(this);
             _connections = new ConcurrentDictionary<HttpConnection, object>();
             _ctxQueue = new ConcurrentDictionary<string, HttpListenerContext>();
         }
-        
+
         /// <inheritdoc />
         public bool IgnoreWriteExceptions { get; set; } = true;
 
@@ -55,6 +58,8 @@ public HttpListener(X509Certificate? certificate = null)
         /// The certificate.
         /// </value>
         internal X509Certificate? Certificate { get; }
+        
+        internal RemoteCertificateValidationCallback? ClientCertificateValidationCallback { get; }
 
         /// <inheritdoc />
         public void Start()
diff --git a/src/EmbedIO/Net/Internal/HttpConnection.cs b/src/EmbedIO/Net/Internal/HttpConnection.cs
index 230a46de..3ce2fe52 100644
--- a/src/EmbedIO/Net/Internal/HttpConnection.cs
+++ b/src/EmbedIO/Net/Internal/HttpConnection.cs
@@ -3,7 +3,7 @@
 using System.Net;
 using System.Net.Security;
 using System.Net.Sockets;
-using System.Security.Cryptography.X509Certificates;
+using System.Security.Authentication;
 using System.Text;
 using System.Threading;
 using System.Threading.Tasks;
@@ -42,14 +42,16 @@ public HttpConnection(Socket sock, EndPointListener epl)
             Stream = new NetworkStream(sock, false);
             if (IsSecure)
             {
-                var sslStream = new SslStream(Stream, true);
+                var sslStream = new SslStream(Stream, true, epl.Listener.ClientCertificateValidationCallback);
 
                 try
                 {
-                    sslStream.AuthenticateAsServer(epl.Listener.Certificate);
+                    var checkClientCertificate = epl.Listener.ClientCertificateValidationCallback != null;
+                    sslStream.AuthenticateAsServer(epl.Listener.Certificate, checkClientCertificate, SslProtocols.None, false);
                 }
-                catch
+                catch (Exception e)
                 {
+                    Console.Error.WriteLine(e);
                     CloseSocket();
                     throw;
                 }
diff --git a/src/EmbedIO/Net/Internal/HttpListenerRequest.cs b/src/EmbedIO/Net/Internal/HttpListenerRequest.cs
index 548f44d7..420dd466 100644
--- a/src/EmbedIO/Net/Internal/HttpListenerRequest.cs
+++ b/src/EmbedIO/Net/Internal/HttpListenerRequest.cs
@@ -4,7 +4,7 @@
 using System.IO;
 using System.Linq;
 using System.Net;
-using System.Security.Cryptography.X509Certificates;
+using System.Net.Security;
 using System.Text;
 using EmbedIO.Internal;
 using EmbedIO.Utilities;
@@ -90,7 +90,7 @@ public Encoding ContentEncoding
         public Stream InputStream => _inputStream ??= ContentLength64 > 0 ? _connection.GetRequestStream(ContentLength64) : Stream.Null;
 
         /// <inheritdoc />
-        public bool IsAuthenticated => false;
+        public bool IsAuthenticated => _connection.Stream is SslStream sslStream && sslStream.IsMutuallyAuthenticated;
 
         /// <inheritdoc />
         public bool IsLocal => LocalEndPoint.Address?.Equals(RemoteEndPoint.Address) ?? true;
diff --git a/src/EmbedIO/WebServer.cs b/src/EmbedIO/WebServer.cs
index f68382d9..db1790d2 100644
--- a/src/EmbedIO/WebServer.cs
+++ b/src/EmbedIO/WebServer.cs
@@ -167,8 +167,8 @@ private IHttpListener CreateHttpListener()
             IHttpListener DoCreate() => Options.Mode switch {
                 HttpListenerMode.Microsoft => System.Net.HttpListener.IsSupported 
                     ? new SystemHttpListener(new System.Net.HttpListener()) as IHttpListener 
-                    : new Net.HttpListener(Options.Certificate),
-                _ => new Net.HttpListener(Options.Certificate)
+                    : new Net.HttpListener(Options.Certificate, Options.ClientCertificateValidationCallback),
+                _ => new Net.HttpListener(Options.Certificate, Options.ClientCertificateValidationCallback)
             };
 
             var listener = DoCreate();
diff --git a/src/EmbedIO/WebServerOptions.cs b/src/EmbedIO/WebServerOptions.cs
index 2700a9f5..c797a618 100644
--- a/src/EmbedIO/WebServerOptions.cs
+++ b/src/EmbedIO/WebServerOptions.cs
@@ -3,6 +3,7 @@
 using System.Diagnostics;
 using System.Globalization;
 using System.Linq;
+using System.Net.Security;
 using System.Security.Cryptography.X509Certificates;
 using System.Text;
 using System.Text.RegularExpressions;
@@ -35,6 +36,8 @@ public sealed class WebServerOptions : WebServerOptionsBase
 
         private StoreLocation _storeLocation = StoreLocation.LocalMachine;
 
+        private RemoteCertificateValidationCallback _clientCertificateValidationCallback;
+
         /// <summary>
         /// Gets the URL prefixes.
         /// </summary>
@@ -169,6 +172,20 @@ public StoreLocation StoreLocation
             }
         }
 
+        /// <summary>
+        /// Gets or sets a callback to validate client-side certificates.
+        /// Client-certificate validation requires SSL to be enabled
+        /// </summary>
+        public RemoteCertificateValidationCallback ClientCertificateValidationCallback
+        {
+            get => _clientCertificateValidationCallback;
+            set
+            {
+                EnsureConfigurationNotLocked();
+                _clientCertificateValidationCallback = value;
+            }
+        }
+
         /// <summary>
         /// Adds a URL prefix.
         /// </summary>
diff --git a/src/EmbedIO/WebServerOptionsExtensions.cs b/src/EmbedIO/WebServerOptionsExtensions.cs
index b68b28d8..454e4d66 100644
--- a/src/EmbedIO/WebServerOptionsExtensions.cs
+++ b/src/EmbedIO/WebServerOptionsExtensions.cs
@@ -1,5 +1,6 @@
 using System;
 using System.Collections.Generic;
+using System.Net.Security;
 using System.Security.Cryptography.X509Certificates;
 using EmbedIO.Utilities;
 
@@ -128,6 +129,21 @@ public static WebServerOptions WithCertificate(this WebServerOptions @this, X509
             @this.Certificate = value;
             return @this;
         }
+        
+        /// <summary>
+        /// Sets the client certificate validation callback delegate
+        /// </summary>
+        /// <param name="this">The <see cref="WebServerOptions"/> on which this method is called.</param>
+        /// <param name="value">The RemoteCertificateValidationCallback to use for mutual SSL connections.</param>
+        /// <returns><paramref name="this"/> with its <see cref="WebServerOptions.ClientCertificateValidationCallback">ClientCertificateValidationCallback</see> property
+        /// set to <paramref name="value"/>.</returns>
+        /// <exception cref="NullReferenceException"><paramref name="this"/> is <see langword="null"/>.</exception>
+        /// <exception cref="InvalidOperationException">The configuration of <paramref name="this"/> is locked.</exception>
+        public static WebServerOptions WithClientCertificateValidation(this WebServerOptions @this, RemoteCertificateValidationCallback value)
+        {
+            @this.ClientCertificateValidationCallback = value;
+            return @this;
+        }
 
         /// <summary>
         /// Sets the thumbprint of the X.509 certificate to use for SSL connections.
diff --git a/test/EmbedIO.Tests/EmbedIO.Tests.csproj b/test/EmbedIO.Tests/EmbedIO.Tests.csproj
index 1ad58b4a..afbf7fb6 100644
--- a/test/EmbedIO.Tests/EmbedIO.Tests.csproj
+++ b/test/EmbedIO.Tests/EmbedIO.Tests.csproj
@@ -22,4 +22,8 @@
     <ProjectReference Include="..\..\src\EmbedIO.Testing\EmbedIO.Testing.csproj" />
   </ItemGroup>
 
+  <ItemGroup>
+    <Content Include="ssl\**" CopyToOutputDirectory="Always" />
+  </ItemGroup>
+
 </Project>
diff --git a/test/EmbedIO.Tests/HttpsTest.cs b/test/EmbedIO.Tests/HttpsTest.cs
index 04b738d2..f4631a66 100644
--- a/test/EmbedIO.Tests/HttpsTest.cs
+++ b/test/EmbedIO.Tests/HttpsTest.cs
@@ -15,6 +15,10 @@ public class HttpsTest
         private const string DefaultMessage = "HOLA";
         private const string HttpsUrl = "https://localhost:5555";
 
+        private const string CLIENT_ONE_CERT_HASH = "7b49b5d24ea5ae856572fd4f103ffbc4443399ce";
+        private const string CLIENT_TWO_CERT_HASH = "a463dcf86fb1daac02a86cda5f9fea7579b8fffb";
+        private const string SERVER_CERT_HASH = "435bb9b0dd3b167c9db218572a6817d01b86f45b";
+
         [Test]
         [Platform("Win")]
         public async Task OpenWebServerHttps_RetrievesIndex()
@@ -71,6 +75,103 @@ public void OpenWebServerHttpsWithInvalidStore_ThrowsInvalidOperation()
 
             Assert.Throws<System.Security.Cryptography.CryptographicException>(() => _ = new WebServer(options));
         }
+        
+        /// <summary>
+        /// Test server with enabled mutual tls authentication for certificate acceptance on both sides
+        /// </summary>
+        [Test]
+        [Platform("Win")]
+        public async Task OpenWebServerHttpsWithClientCertificate_AcceptsKnownCertificate()
+        {
+            var options = new WebServerOptions()
+                .WithUrlPrefix(HttpsUrl)
+                .WithCertificate(new X509Certificate2(@".\ssl\server.pfx", "embedio"))
+                .WithMode(HttpListenerMode.EmbedIO)
+                .WithClientCertificateValidation(
+                    (sender, certificate, chain, errors) => 
+                        certificate == null || CLIENT_ONE_CERT_HASH.Equals(certificate.GetCertHashString(), StringComparison.OrdinalIgnoreCase));
+
+            using var webServer = new WebServer(options);
+            webServer.OnAny(ctx => {
+                Assert.True(ctx.Request.IsAuthenticated, "User is authenticated");
+                return ctx.SendStringAsync(DefaultMessage, MimeType.PlainText, WebServer.DefaultEncoding);
+            });
+
+            _ = webServer.RunAsync();
+
+            using var httpClientHandler = new HttpClientHandler {
+                ServerCertificateCustomValidationCallback = ValidateFixedServerCertificate,
+                ClientCertificateOptions = ClientCertificateOption.Manual,
+                ClientCertificates = { new X509Certificate2(@"ssl/client1.pfx", "embedio") }
+            };
+            using var httpClient = new HttpClient(httpClientHandler);
+            Assert.AreEqual(DefaultMessage, await httpClient.GetStringAsync(HttpsUrl));
+        }
+        
+        /// <summary>
+        /// Test server with enabled mutual tls authentication during a refused mutual authentication on the client side 
+        /// </summary>
+        [Test]
+        [Platform("Win")]
+        public async Task OpenWebServerHttpsWithClientCertificate_CanAcceptAnon()
+        {
+            var options = new WebServerOptions()
+                .WithUrlPrefix(HttpsUrl)
+                .WithCertificate(new X509Certificate2(@".\ssl\server.pfx", "embedio"))
+                .WithMode(HttpListenerMode.EmbedIO)
+                // by treating an missing certificate as okay, we can allow anon requests
+                .WithClientCertificateValidation(
+                    (sender, certificate, chain, errors) => 
+                        certificate == null || CLIENT_ONE_CERT_HASH.Equals(certificate.GetCertHashString(), StringComparison.OrdinalIgnoreCase));
+
+            using var webServer = new WebServer(options);
+            webServer.OnAny(ctx => {
+                // The user did not provide any certificate and thus is treated as anonymous
+                Assert.False(ctx.Request.IsAuthenticated, "User is authenticated");
+                return ctx.SendStringAsync(DefaultMessage, MimeType.PlainText, WebServer.DefaultEncoding);
+            });
+
+            _ = webServer.RunAsync();
+
+            using var httpClientHandler = new HttpClientHandler {
+                ServerCertificateCustomValidationCallback = ValidateFixedServerCertificate
+            };
+            using var httpClient = new HttpClient(httpClientHandler);
+            Assert.AreEqual(DefaultMessage, await httpClient.GetStringAsync(HttpsUrl));
+        }
+        
+        /// <summary>
+        /// Test server with enabled mutual tls authentication when the provided client certificate is not accepted
+        /// </summary>
+        [Test]
+        [Platform("Win")]
+        public async Task OpenWebServerHttpsWithClientCertificate_RejectsUnknownCert()
+        {
+            var options = new WebServerOptions()
+                .WithUrlPrefix(HttpsUrl)
+                .WithCertificate(new X509Certificate2(@".\ssl\server.pfx", "embedio"))
+                .WithMode(HttpListenerMode.EmbedIO)
+                // refuse all certificates to make client certificate validation fail
+                .WithClientCertificateValidation(
+                    (sender, certificate, chain, errors) => false);
+
+            using var webServer = new WebServer(options);
+            webServer.OnAny(ctx => {
+                // The user did not provide any certificate and thus is treated as anonymous
+                Assert.Fail("Server should refuse service");
+                return ctx.SendStringAsync(DefaultMessage, MimeType.PlainText, WebServer.DefaultEncoding);
+            });
+
+            _ = webServer.RunAsync();
+
+            using var httpClientHandler = new HttpClientHandler {
+                ServerCertificateCustomValidationCallback = ValidateFixedServerCertificate,
+                ClientCertificateOptions = ClientCertificateOption.Manual,
+                ClientCertificates = { new X509Certificate2(@"ssl/client2.pfx", "embedio") }
+            };
+            using var httpClient = new HttpClient(httpClientHandler);
+            Assert.ThrowsAsync<HttpRequestException>(async () => await httpClient.GetStringAsync(HttpsUrl));
+        }
 
         // Bypass certificate validation.
         private static bool ValidateCertificate(object sender,
@@ -78,5 +179,10 @@ private static bool ValidateCertificate(object sender,
                                                 X509Chain chain,
                                                 SslPolicyErrors sslPolicyErrors)
             => true;
+
+        private static bool ValidateFixedServerCertificate(HttpRequestMessage message, X509Certificate2? certificate, X509Chain? chain, SslPolicyErrors errors)
+        {
+            return certificate != null && SERVER_CERT_HASH.Equals(certificate.GetCertHashString(), StringComparison.OrdinalIgnoreCase);
+        }
     }
 }
\ No newline at end of file