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nextflow.config
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nextflow.config
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/*
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
qbic-pipelines/rnadeseq Nextflow config file
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Default config options for all compute environments
----------------------------------------------------------------------------------------
*/
// Global default params, used in configs
// params marked with //* exist for the sake of completeness only;
// enabling them prevents igenomes from working
params {
// Main input args
input_type = 'featurecounts'
gene_counts = null
input = null
model = null
//* gtf = null
// DESeq2 args
contrast_matrix = 'DEFAULT'
contrast_list = 'DEFAULT1'
contrast_pairs = 'DEFAULT2'
genelist = 'NO_FILE'
relevel = 'NO_FILE2'
batch_effect = false
logFC_threshold = 0
adj_pval_threshold = 0.05
norm_method = 'rlog'
vst_genes_number = 1000
round_DE = -1
// Pathway analysis args
//* organism = null
//* species_library = null
//* keytype = null
run_pathway_analysis = false
min_DEG_pathway = 1
custom_gmt = 'NO_FILE3'
set_background = true
custom_background = 'NO_FILE7'
datasources = null
heatmaps_cluster_rows = true
heatmaps_cluster_cols = false
// Additional args for the report
project_summary = null
multiqc = 'NO_FILE4'
quote = 'NO_FILE5'
software_versions = 'NO_FILE6'
report_file = "$projectDir/assets/RNAseq_report.Rmd"
references_file = "$projectDir/assets/references.bibtex"
css = "$projectDir/assets/corp-styles.css"
logo = "$projectDir/assets/logo.png"
// Additional arg to indicate that a github test is run
citest = false
// Reference genome options
genome = null
igenomes_base = 's3://ngi-igenomes/igenomes'
igenomes_ignore = false
// nextflow options
publish_dir_mode = null
outdir = './results'
// Boilerplate options
email = null
email_on_fail = null
plaintext_email = false
monochrome_logs = false
help = false
tracedir = "${params.outdir}/pipeline_info"
custom_config_version = 'master'
custom_config_base = "https://raw.githubusercontent.com/nf-core/configs/${params.custom_config_version}"
config_profile_description = null
config_profile_contact = null
config_profile_url = null
hook_url = null
// Max resource options
// Defaults only, expecting to be overwritten
max_memory = '128.GB'
max_cpus = 16
max_time = '240.h'
// Schema validation default options (only those necessary for linting; rnadeseq does not use the validation plugin)
validationSchemaIgnoreParams = 'genomes,igenomes_base'
validationShowHiddenParams = false
}
// Load base.config by default for all pipelines
includeConfig 'conf/base.config'
// Load nf-core custom profiles from different Institutions
try {
includeConfig "${params.custom_config_base}/nfcore_custom.config"
} catch (Exception e) {
System.err.println("WARNING: Could not load nf-core/config profiles: ${params.custom_config_base}/nfcore_custom.config")
}
// Load qbic-pipelines/rnadeseq custom profiles from different institutions.
// Warning: Uncomment only if a pipeline-specific instititutional config already exists on nf-core/configs!
// try {
// includeConfig "${params.custom_config_base}/pipeline/rnadeseq.config"
// } catch (Exception e) {
// System.err.println("WARNING: Could not load nf-core/config/rnadeseq profiles: ${params.custom_config_base}/pipeline/rnadeseq.config")
// }
profiles {
debug {
dumpHashes = true
process.beforeScript = 'echo $HOSTNAME'
cleanup = false
nextflow.enable.configProcessNamesValidation = true
}
conda {
conda.enabled = true
docker.enabled = false
singularity.enabled = false
podman.enabled = false
shifter.enabled = false
charliecloud.enabled = false
conda.channels = ['conda-forge', 'bioconda', 'defaults']
apptainer.enabled = false
}
mamba {
conda.enabled = true
conda.useMamba = true
docker.enabled = false
singularity.enabled = false
podman.enabled = false
shifter.enabled = false
charliecloud.enabled = false
apptainer.enabled = false
}
docker {
docker.enabled = true
conda.enabled = false
singularity.enabled = false
podman.enabled = false
shifter.enabled = false
charliecloud.enabled = false
apptainer.enabled = false
docker.runOptions = '-u $(id -u):$(id -g)'
}
arm {
docker.runOptions = '-u $(id -u):$(id -g) --platform=linux/amd64'
}
singularity {
singularity.enabled = true
singularity.autoMounts = true
conda.enabled = false
docker.enabled = false
podman.enabled = false
shifter.enabled = false
charliecloud.enabled = false
apptainer.enabled = false
}
podman {
podman.enabled = true
conda.enabled = false
docker.enabled = false
singularity.enabled = false
shifter.enabled = false
charliecloud.enabled = false
apptainer.enabled = false
}
shifter {
shifter.enabled = true
conda.enabled = false
docker.enabled = false
singularity.enabled = false
podman.enabled = false
charliecloud.enabled = false
apptainer.enabled = false
}
charliecloud {
charliecloud.enabled = true
conda.enabled = false
docker.enabled = false
singularity.enabled = false
podman.enabled = false
shifter.enabled = false
apptainer.enabled = false
}
apptainer {
apptainer.enabled = true
apptainer.autoMounts = true
conda.enabled = false
docker.enabled = false
singularity.enabled = false
podman.enabled = false
shifter.enabled = false
charliecloud.enabled = false
}
wave {
apptainer.ociAutoPull = true
singularity.ociAutoPull = true
wave.enabled = true
wave.freeze = true
wave.strategy = 'conda,container'
}
gitpod {
executor.name = 'local'
executor.cpus = 4
executor.memory = 8.GB
}
test { includeConfig 'conf/test.config' }
test_full { includeConfig 'conf/test_full.config' }
test_contrast_matrix { includeConfig 'conf/test_contrast_matrix.config' }
test_contrast_list { includeConfig 'conf/test_contrast_list.config' }
test_relevel { includeConfig 'conf/test_relevel.config' }
test_use_vst { includeConfig 'conf/test_use_vst.config' }
test_smrnaseq { includeConfig 'conf/test_smrnaseq.config' }
test_star_salmon { includeConfig 'conf/test_star_salmon.config' }
test_star_rsem { includeConfig 'conf/test_star_rsem.config' }
test_batcheffect { includeConfig 'conf/test_batcheffect.config' }
test_no_multiqc { includeConfig 'conf/test_no_multiqc.config' }
test_custom_gmt { includeConfig 'conf/test_custom_gmt.config' }
}
// Set default registry for Apptainer, Docker, Podman and Singularity independent of -profile
// Will not be used unless Apptainer / Docker / Podman / Singularity are enabled
// Set to your registry if you have a mirror of containers
apptainer.registry = 'quay.io'
docker.registry = 'quay.io'
podman.registry = 'quay.io'
singularity.registry = 'quay.io'
// Load igenomes.config if required
if (!params.igenomes_ignore) {
includeConfig 'conf/igenomes.config'
} else {
params.genomes = [:]
}
// Export these variables to prevent local Python/R libraries from conflicting with those in the container
// The JULIA depot path has been adjusted to a fixed path `/usr/local/share/julia` that needs to be used for packages in the container.
// See https://apeltzer.github.io/post/03-julia-lang-nextflow/ for details on that. Once we have a common agreement on where to keep Julia packages, this is adjustable.
env {
PYTHONNOUSERSITE = 1
R_PROFILE_USER = "/.Rprofile"
R_ENVIRON_USER = "/.Renviron"
JULIA_DEPOT_PATH = "/usr/local/share/julia"
}
// Capture exit codes from upstream processes when piping
process.shell = ['/bin/bash', '-euo', 'pipefail']
// Disable process selector warnings by default. Use debug profile to enable warnings.
nextflow.enable.configProcessNamesValidation = false
def trace_timestamp = new java.util.Date().format( 'yyyy-MM-dd_HH-mm-ss')
timeline {
enabled = true
file = "${params.outdir}/pipeline_info/execution_timeline_${trace_timestamp}.html"
}
report {
enabled = true
file = "${params.outdir}/pipeline_info/execution_report_${trace_timestamp}.html"
}
trace {
enabled = true
file = "${params.outdir}/pipeline_info/execution_trace_${trace_timestamp}.txt"
}
dag {
enabled = true
file = "${params.outdir}/pipeline_info/pipeline_dag_${trace_timestamp}.html"
}
manifest {
name = 'qbic-pipelines/rnadeseq'
author = """Gisela Gabernet, Silvia Morini, Oskar Wacker, QBiC"""
homePage = 'https://github.com/qbic-pipelines/rnadeseq'
description = """Differential gene expression analysis and pathway analysis of RNAseq data"""
mainScript = 'main.nf'
nextflowVersion = '!>=23.04.0'
version = '2.4'
doi = ''
}
// Load modules.config for DSL2 module specific options
includeConfig 'conf/modules.config'
// Function to ensure that resource requirements don't go beyond
// a maximum limit
def check_max(obj, type) {
if (type == 'memory') {
try {
if (obj.compareTo(params.max_memory as nextflow.util.MemoryUnit) == 1)
return params.max_memory as nextflow.util.MemoryUnit
else
return obj
} catch (all) {
println " ### ERROR ### Max memory '${params.max_memory}' is not valid! Using default value: $obj"
return obj
}
} else if (type == 'time') {
try {
if (obj.compareTo(params.max_time as nextflow.util.Duration) == 1)
return params.max_time as nextflow.util.Duration
else
return obj
} catch (all) {
println " ### ERROR ### Max time '${params.max_time}' is not valid! Using default value: $obj"
return obj
}
} else if (type == 'cpus') {
try {
return Math.min( obj, params.max_cpus as int )
} catch (all) {
println " ### ERROR ### Max cpus '${params.max_cpus}' is not valid! Using default value: $obj"
return obj
}
}
}