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fasta_3.4(t25d6).yaml
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fasta_3.4(t25d6).yaml
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!mobyle/program
name: fasta
version: 3.4(t25d6)
title: FASTA
description: Sequence database search
authors: W. Pearson
inputs: !mobyle/inputparagraph
children:
- !mobyle/inputprogramparameter
comment: '- fasta: scan a protein or DNA sequence library for similar sequences-
tfasta: compare a protein sequence to a DNA sequence librarSy, translating
the DNA sequence library `on-the-fly'' to the 3 forward and the 3 reverse
frames without frameshifts.- fastx/fasty: compare a DNA sequence to a
protein sequence database, comparing the translated DNA sequence in three
frames, with frameshifts. fasty2 allows frameshifts inside codons.- tfastx/tfasty:
compare a protein sequence vs a translated DNA db, with frameshifts. tfasty
allows frameshifts inside codons.- fastf/tfastf: compare an ordered peptide
mixture (obtained for example by Edman degradation of a CNBr cleavage)
against a protein or translated DNA database.- fasts/tfasts: compare a
set of short peptide fragments (obtained from a mass-spec analysis of
a protein) against a protein or translated DNA database.'
prompt: Fasta program
format: str(value) + " -q"
simple: true
argpos: 0
mandatory: true
name: fasta
command: true
type: !mobyle/stringtype
default: 'null'
options:
- {label: Choose a program, value: 'null'}
- {label: fasta (protein or nucleotide query vs similar db ), value: fasta}
- {label: tfasta (protein query vs translated nucleic db), value: tfasta}
- {label: fastx (translated nucleotide query vs protein db (frameshifts
only only between codons)), value: fastx}
- {label: tfastx (protein query vs translated DNA db (frameshifts only
between codons)), value: tfastx}
- {label: fasty (fastx + frameshifts anywhere), value: fasty}
- {label: tfasty (tfastx + frameshifts anywhere), value: tfasty}
- {label: fastf (mixed peptide seq vs protein db (modified algorithm)),
value: fastf}
- {label: tfastf (mixed peptide seq vs translated DNA db (modified algorithm)),
value: tfastf}
- {label: fasts (several short peptide seq vs protein db (modified algorithm)),
value: fasts}
- {label: tfasts (several short peptide seq vs translated DNA db (modified
algorithm)), value: tfasts}
- !mobyle/inputprogramparameter
prompt: Query sequence File
format: '" "+str(value)'
simple: true
argpos: 2
mandatory: true
name: query
command: false
type: !mobyle/formattedtype
format_terms: ['EDAM_format:2200']
data_terms: ['EDAM_data:2044']
- !mobyle/inputprogramparameter
prompt: Is it a DNA or protein sequence (-n)
format: ( "" , " -n" )[ value is not None and value == "DNA" and fasta ==
'fasta']
simple: true
argpos: 1
mandatory: true
name: seqtype
command: false
type: !mobyle/stringtype
default: 'null'
options:
- {label: Choose a biotype, value: 'null'}
- {label: DNA, value: DNA}
- {label: Protein, value: protein}
- !mobyle/inputprogramparagraph
prompt: Database
name: db
argpos: 3
children:
- !mobyle/inputprogramparameter
comment: Choose a protein db for fasta, fastx, fatsf, fasty or fasts.Please
note that Swissprot usage by and for commercial entities requires
a license agreement.
prompt: Protein Database
format: '" /path/to/fasta/databank/dir/" + str(value)'
simple: true
mandatory: true
name: protein_db
precond:
'#or':
- '#and':
- {seqtype: protein}
- {fasta: fasta}
- fasta:
'#in': [fastx, fasty, fastf, fasts]
command: false
type: !mobyle/stringtype
default: 'null'
options:
- {label: Choose a database, value: 'null'}
- {label: 'uniprot: Universal Protein Resource', value: uniprot}
- {label: 'uniprot_sprot: Universal Protein Resource (SwissProt part)',
value: uniprot_sprot}
- {label: 'uniprot_trembl: Universal Protein Resource (TrEmbl part)',
value: uniprot_trembl}
- {label: 'nrprot: NCBI non-redundant Genbank CDS translations+PDB+Swissprot+PIR',
value: nrprot}
- {label: 'nrprot_month: NCBI month non-redundant Genbank CDS translations+PDB+Swissprot+PIR',
value: nrprot_month}
- {label: 'genpept: Genbank translations (last rel. + upd.)', value: genpept}
- {label: 'genpept_new: genpept updates', value: genpept_new}
- {label: 'gpbct: genpept bacteries', value: gpbct}
- {label: 'gppri: primates', value: gppri}
- {label: 'gpmam: other mammals', value: gpmam}
- {label: 'gprod: rodents', value: gprod}
- {label: 'gpvrt: other vertebrates', value: gpvrt}
- {label: 'gpinv: invertebrates', value: gpinv}
- {label: 'gppln: plants (including yeast)', value: gppln}
- {label: 'gpvrl: virus', value: gpvrl}
- {label: 'gpphg: phages', value: gpphg}
- {label: 'gpsts: STS', value: gpsts}
- {label: 'gpsyn: synthetic', value: gpsyn}
- {label: 'gppat: patented', value: gppat}
- {label: 'gpuna: unatotated', value: gpuna}
- {label: 'gphtg: GS (high throughput Genomic Sequencing)', value: gphtg}
- {label: 'sbase: annotated domains sequences', value: sbase}
- !mobyle/inputprogramparameter
comment: Choose a nucleotide db for fasta, tfasta, tfastx, tfasty, tfastf
or tfasts
prompt: Nucleotid Database
format: '" /path/to/fasta/databank/dir/" + str(value)'
simple: true
mandatory: true
name: nucleotid_db
precond:
'#or':
- '#and':
- {seqtype: DNA}
- {fasta: fasta}
- fasta:
'#in': [tfasta, tfastx, tfasty, tfastf, tfasts]
command: false
type: !mobyle/stringtype
default: 'null'
options:
- {label: Choose a database, value: 'null'}
- {label: 'embl: Embl last release + updates', value: embl}
- {label: 'embl_new: Embl updates', value: embl_new}
- {label: 'genbank: Genbank last release + updates', value: genbank}
- {label: 'genbank_new: Genbank updates', value: genbank_new}
- {label: 'gbbct: genbank bacteria', value: gbbct}
- {label: 'gbpri: primates', value: gbpri}
- {label: 'gbmam: other mammals', value: gbmam}
- {label: 'gbrod: rodents', value: gbrod}
- {label: 'gbvrt: other vertebrates', value: gbvrt}
- {label: 'gbinv: invertebrates', value: gbinv}
- {label: 'gbpln: plants (including yeast)', value: gbpln}
- {label: 'gbvrl: virus', value: gbvrl}
- {label: 'gbphg: phages', value: gbphg}
- {label: 'gbest: EST (Expressed Sequence Tags)', value: gbest}
- {label: 'gbsts: STS (Sequence Tagged sites)', value: gbsts}
- {label: 'gbsyn: synthetic', value: gbsyn}
- {label: 'gbpat: patented', value: gbpat}
- {label: 'gbuna: unannotated', value: gbuna}
- {label: 'gbgss: Genome Survey Sequences', value: gbgss}
- {label: 'gbhtg: GS (high throughput Genomic Sequencing)', value: gbhtg}
- {label: 'imgt: IMGT/LIGM-DB, ImMunoGeneTics sequence database',
value: imgt}
- {label: 'borrelia: Borrelia burgdorferi complete genome', value: borrelia}
- {label: 'ecoli: Escherichia Coli complete genome', value: ecoli}
- {label: 'genitalium: Mycoplasma Genitalium complete genome', value: genitalium}
- {label: 'pneumoniae: Mycoplasma Pneumoniae complete genome', value: pneumoniae}
- {label: 'hpylori: Helicobacter Pylori complete genome', value: hpylori}
- {label: 'bsubtilis: Bacillus Subtilis complete genome', value: bsubtilis}
- {label: 'tuberculosis: Mycobacterium tuberculosis complete genome',
value: tuberculosis}
- {label: 'ypestis: Yersinia pestis unfinished genome', value: ypestis}
- {label: 'yeast: Yeast chromosomes', value: yeast}
- {label: 'pfalciparum: Plasmodium falciparum 3D7', value: pfalciparum}
- !mobyle/inputprogramparameter
comment: Break long library sequences into blocks of N residues. Useful
for bacterial genomes, which have only one sequence entry. -N 2000
works well for well for bacterial genomes.
prompt: Break long library sequences into blocks (-N)
format: ( "" , " -N " + str(value) )[ value is not None ]
name: break_long
command: false
type: !mobyle/integertype {}
- !mobyle/inputprogramparagraph
prompt: Selectivity options
name: selectivity_opt
argpos: 1
children:
- !mobyle/inputprogramparameter
comment: ktup sets the sensitivity and speed of the search. If ktup=2,
similar regions in the two sequences being compared are found by looking
at pairs of aligned residues; if ktup=1, single aligned amino acids
are examined. ktup can be set to 2 or 1 for protein sequences, or
from 1 to 6 for DNA sequences. The default if ktup is not specified
is 2 for proteins and 6 for DNA. 1ktup=1 should be used for oligonucleotides
(DNA query length < 20).
prompt: Sensitivity and speed of the search
format: ("" , " " + str(value) )[ value is not None ]
argpos: 4
name: ktup
command: false
type: !mobyle/integertype {}
- !mobyle/inputprogramparameter
comment: The threshold value is normally calculated based on sequence
length.
prompt: Threshold for band optimization (FASTA, FASTX). (-c)
format: ("" , " -c " + str(value) )[ value is not None ]
name: optcut
command: false
type: !mobyle/integertype {}
- !mobyle/inputprogramparameter
comment: The default for fasta with proteins is -12 and -16 for DNAThe
default for fastx/fasty/tfastz/tfasty is -15.
prompt: Penalty for opening a gap (-f)
format: ("" , " -f " + str(value))[ value is not None ]
name: gapinit
command: false
type: !mobyle/integertype {}
- !mobyle/inputprogramparameter
comment: The default for fasta is -2 for proteins and -4 for DNAThe default
for fastx/fasty/tfastz/tfasty is -3.
prompt: Penalty for gap extension (-g)
format: ("" , " -g " + str(value))[ value is not None ]
name: gapext
command: false
type: !mobyle/integertype {}
- !mobyle/inputprogramparameter
comment: Expectation value limit for displaying scores and alignments.
Defaults are 10.0 for FASTA protein searches, 5.0 for translated
DNA/protein comparisons, and 2.0 for DNA/DNA searches.
prompt: Maximal expectation value threshold for displaying scores and
alignments (-E)
format: ("" , " -E " + str(value))[ value is not None and value != vdef]
name: high_expect
command: false
type: !mobyle/floattype {default: 10.0}
- !mobyle/inputprogramparameter
comment: Expectation value lower limit for score and alignment display. If
value is 1e-6 prevents library sequences with E()-values lower than
1e-6 from being displayed. This allows the use to focus on more distant
relationships.This allow one to skip over close relationships in searches
for more distant relationships.
prompt: Minimal expectation value threshold for displaying scores and
alignments (-F)
format: ("" , " -F " + str(value))[ value is not None ]
name: low_expect
command: false
type: !mobyle/floattype {}
- !mobyle/inputprogramparagraph
prompt: Scoring options
name: score_opt
argpos: 1
children:
- !mobyle/inputprogramparagraph
prompt: Nucleic penalty
name: scoring_nucleic
precond:
'#and':
- {fasta: fasta}
- {seqtype: DNA}
children:
- !mobyle/inputprogramparameter
prompt: Maximum positive value for a nucleotid match (-r)
name: nucleotid_match
command: false
type: !mobyle/integertype {default: 5}
- !mobyle/inputprogramparameter
comment: '''+5/-4'' are the default values for nucleotid match/mismatch,
but ''+3/-2'' can perform better in some cases.'
prompt: Maximum negative penalty value for a nucleotid mismatch (-r)
format: ( "" , ' -r "' + str(nucleotid_match) + '/' + str(value)+
'"' )[ value is not None and nucleotid_match is not None and (nucleotid_match
!= 5 and value != vdef) ]
name: nucleotid_mismatch
precond:
nucleotid_match: {'#ne': None}
command: false
type: !mobyle/integertype {default: -4}
- !mobyle/inputprogramparagraph
prompt: Protein penalty
name: scoring_protein
precond:
seqtype: {'#ne': DNA}
children:
- !mobyle/inputprogramparameter
prompt: Scoring matrix file (-s)
format: ( "" , " -s " + str(value) )[ value is not None and value
!= vdef]
name: matrix
command: false
type: !mobyle/stringtype
default: BL50
options:
- {label: BLOSUM50, value: BL50}
- {label: BLOSUM62, value: BL62}
- {label: BLOSUM80, value: BL80}
- {label: PAM20, value: P20}
- {label: PAM40, value: P40}
- {label: PAM120, value: P120}
- {label: PAM250, value: P250}
- {label: MDM_10, value: M10}
- {label: MDM_20, value: M20}
- {label: MDM_40, value: M40}
- !mobyle/inputprogramparameter
comment: Particularly useful for fast[xy], where termination codons
are encoded as 'X'.
prompt: Penalty for a match to 'X' (independently of the PAM matrix)
(-x)
format: ( "" , " -x " + str(value) )[ value is not None ]
name: X_penalty
command: false
type: !mobyle/integertype {}
- !mobyle/inputprogramparagraph
prompt: Frameshift and translation options
name: frame_transl_opt
argpos: 1
children:
- !mobyle/inputprogramparameter
prompt: Penalty for frameshift between two codons (fast[xy]/tfast[xy])
(-h)
format: ("" , " -h " + str(value))[ value is not None ]
name: frameshift
precond:
fasta:
'#in': [fastx, fasty, tfastx, tfasty]
command: false
type: !mobyle/integertype {}
- !mobyle/inputprogramparameter
prompt: Penalty for frameshift within a codon (fasty/tfasty) (-j)
format: ("" , " -j " + str(value))[ value is not None ]
name: frameshift_within
precond:
fasta:
'#in': [fasty, tfasty]
command: false
type: !mobyle/integertype {}
- !mobyle/inputprogramparameter
prompt: Search only the three forward frames (-3)
format: ("" , " -3")[ value ]
name: threeframe
precond:
fasta:
'#in': [tfasta, tfastx, tfasty]
command: false
type: !mobyle/booleantype {default: false}
- !mobyle/inputprogramparameter
prompt: Reverse complement the query sequence (-i)
format: ( "" , " -i" )[ value ]
name: invert
precond:
fasta:
'#in': [fastx, tfastx, fasty, tfasty]
command: false
type: !mobyle/booleantype {default: false}
- !mobyle/inputprogramparameter
prompt: Use genetic code for translation (tfasta/tfast[xy]/fast[xy]) (-t)
format: ( "" , " -t " + str(value) )[ value is not None and value !=
vdef]
name: genetic_code
precond:
fasta:
'#in': [tfasta, tfastx, tfasty, tfastf, tfasts, fastx, fasty]
command: false
type: !mobyle/stringtype
default: '1'
options:
- {label: Standard (1), value: '1'}
- {label: Vertebrate Mitochondrial (1), value: '2'}
- {label: Yeast Mitochondrial (2), value: '3'}
- {label: 'Mold, Protozoan, Coelenterate Mitochondrial and Mycoplasma/Spiroplasma
(3)', value: '4'}
- {label: Invertebrate Mitochondrial (4), value: '5'}
- {label: Ciliate Macronuclear and Dasycladacean (5), value: '6'}
- {label: Echinoderm Mitochondrial (6), value: '9'}
- {label: Euplotid Nuclear (7), value: '10'}
- {label: Bacterial (8), value: '11'}
- {label: Alternative Yeast Nuclear (9), value: '12'}
- {label: Ascidian Mitochondrial (10), value: '13'}
- {label: Flatworm Mitochondrial (11), value: '14'}
- {label: Blepharisma Macronuclear (12), value: '15'}
- !mobyle/inputprogramparagraph
prompt: Optimization options
name: optimize_opt
argpos: 1
children:
- !mobyle/inputprogramparameter
comment: Set the band-width used for optimization. -y 16 is the default
for protein when ktup=2 and for all DNA alignments. -y 32 is used
for protein and ktup=1. For proteins, optimization slows comparison
2-fold and is highly recommended.
prompt: Band-width used for optimization (-y)
format: ("" , " -y " + str(value))[ value is not None ]
name: band
command: false
type: !mobyle/integertype {}
- !mobyle/inputprogramparameter
comment: Force Smith-Waterman alignment for output. Smith-Waterman is
the default for protein sequences and FASTX, but not for TFASTA or
DNA comparisons with FASTA.
prompt: Force Smith-Waterman alignment for DNA (-A)
format: ("" , " -A")[ value ]
name: swalig
precond:
'#and':
- fasta:
'#in': [tfasta, fasta]
- {seqtype: DNA}
command: false
type: !mobyle/booleantype {default: false}
- !mobyle/inputprogramparameter
comment: Turn off default optimization of all scores greater than OPTCUT.
Shirt results by 'initn' scores reduces the accuracy of statistical
estimates. This was the behavior of fasta1 versions.
prompt: Turn fasta band optimization off during initial phase (-o)
format: ("" , " -o")[ value ]
name: noopt
command: false
type: !mobyle/booleantype {default: false}
- !mobyle/inputprogramparameter
comment: In general, 1 and 2 are the best methods.
prompt: Specify statistical calculation. (-z)
format: ( (( "", " -z " + str(value) ) [ value is not None and value
!= vdef]) , " -z 1" + str(value) )[value is not None and value > 0
and random is not None ]
name: stat
command: false
type: !mobyle/stringtype
default: '1'
options:
- {label: Turn off statistics (0), value: '0'}
- {label: Weigthed regression against the length of the library sequence
(1), value: '1'}
- {label: Maximum likelihood estimates of Lambda abd K (2), value: '2'}
- {label: Altschul-Gishas statistical (3), value: '3'}
- {label: 'Alternate regression method: Variation 1 of 1 (4)', value: '4'}
- {label: 'Alternate regression method: Variation 2 of 1 (5)', value: '5'}
- {label: Maximum likelihood estimate based on the method of Mott
(6), value: '6'}
- !mobyle/inputprogramparameter
comment: This doubles the time required for a search, but allows accurate
statistics to be estimated for libraries comprised of a single protein
family.
prompt: Estimate statistical parameters from shuffled copies of each library
sequence (-z)
name: random
precond:
stat: {'#gt': '0'}
command: false
type: !mobyle/booleantype {default: false}
- !mobyle/inputprogramparagraph
prompt: Report options
name: affichage
argpos: 1
children:
- !mobyle/inputprogramparameter
prompt: Turn off histogram display (-H)
format: ("" , " -H" )[ value ]
name: histogram
command: false
type: !mobyle/booleantype {default: false}
- !mobyle/inputprogramparameter
prompt: Number of similarity scores to be shown (-b)
format: ("" , " -b " + str(value))[ value is not None and value <= high_expect]
name: scores
command: false
type: !mobyle/integertype {}
- !mobyle/inputprogramparameter
prompt: Number of alignments to be shown (-d)
format: ("" , " -d " + str(value))[ value is not None and value <= high_expect]
name: alns
command: false
type: !mobyle/integertype {}
- !mobyle/inputprogramparameter
prompt: HTML output (-m)
format: ( "" , " -m 6" )[ value ]
simple: true
name: html_output
command: false
type: !mobyle/booleantype {default: false}
- !mobyle/inputprogramparameter
comment: (MARKX) =0,1,2,3,4. Alternate display of matches and mismatches
in alignments.MARKX=0 uses ':','.',' ', for identities, conservative
replacements, and non-conservative replacements, respectively.MARKX=1
uses ' ','x', and 'X'.MARKX=2 does not show the second sequence, but
uses the second alignment line to display matches with a '.' for identity,
or with the mismatched residue for mismatches. MARKX=2 is useful for
aligning large numbers of similar sequences.MARKX=3 writes out a file
of library sequences in FASTA format. MARKX=3 should always be used
with the 'SHOWALL' (-a) option, but this does not completely ensure
that all of the sequences output will be aligned.MARKX=4 displays
a graph of the alignment of the library sequence with respect to the
query sequence, so that one can identify the regions of the query
sequence that are conserved.
prompt: Alternate display of matches and mismatches in alignments (-m)
format: ( "" , " -m " + str(value) )[ value is not None and value !=
vdef]
name: markx
precond: {'#not': html_output}
command: false
type: !mobyle/stringtype
default: '0'
options:
- {label: '0 [: identities] [. conservative repl] [ non-conserv repl]',
value: '0'}
- {label: '1: [ identities] [x conservative repl] [X non-conserv repl]',
value: '1'}
- {label: '2: [. identities] [res mismatch] - don''t display the 2nd
seq', value: '2'}
- {label: '3: writes a file of library sequences in FASTA format',
value: '3'}
- {label: '4: displays a graph of the alignment', value: '4'}
- {label: '9: 0 + percent identity + coordinates', value: '9'}
- {label: '10: output more information', value: '10'}
- !mobyle/inputprogramparameter
prompt: Sequences ranked by the z-score based on the init1 score (-1)
format: ("" , " -1")[ value ]
name: init1
command: false
type: !mobyle/booleantype {default: false}
- !mobyle/inputprogramparameter
prompt: Show normalize score as (-B)
format: ( "" , " -B" )[ value is not None and value != vdef]
name: z_score_out
command: false
type: !mobyle/stringtype
default: '0'
options:
- {label: z-score (1), value: '1'}
- {label: bit-score (0), value: '0'}
- !mobyle/inputprogramparameter
prompt: Output line length for sequence alignments (-w)
format: ("" , " -w " + str(value))[ value is not None and value != vdef
]
name: linlen
command: false
type: !mobyle/integertype {default: 60}
- !mobyle/inputprogramparameter
comment: Causes fasta/lfasta/plfasta to start numbering the aligned sequences
starting with offset1 and offset2, rather than 1 and 1. This is particularly
useful for showing alignments of promoter regions.
prompt: Start numbering the aligned sequences at position x1 x2 (2 numbers
separated by comma) (-X)
format: ("" , ' -X "' + str(value) + '"')[ value is not None ]
name: offsets
command: false
type: !mobyle/stringtype {}
- !mobyle/inputprogramparameter
prompt: Display more information about the library sequence in the alignment
(-L)
format: ("" , " -L")[ value ]
name: info
command: false
type: !mobyle/booleantype {default: false}
- !mobyle/inputprogramparagraph
prompt: Other options
name: other_opt
argpos: 1
children:
- !mobyle/inputprogramparameter
comment: Treat lower-case characters in the query or library sequence
as 'low-complexity' residues. These characters are treated as 'X'
during the initial scan, but are treated as normal residues during
the final alignment. Sinces statistical significance is calculated
from similarity score calculated during library search, low complexity
regions will not produce statistical significant matches.If a significant
alignment contains low complexity regions the final score may be higher
than the score obtained during the search.
prompt: Lower case filtering (-S)
format: ( "" , " -S" )[ value ]
name: filter
command: false
type: !mobyle/booleantype {default: false}
outputs: !mobyle/outputparagraph
children:
- !mobyle/outputprogramparameter
prompt: Fasta report
filenames: '"fasta.out"'
name: outfile
output_type: stdout
type: !mobyle/formattedtype
data_terms: ['EDAM_data:2048']
- !mobyle/outputprogramparameter
prompt: Html output file
filenames: '"fasta.html"'
argpos: 100
name: html_outfile
precond: html_output
type: !mobyle/formattedtype
data_terms: ['EDAM_data:2048']
- !mobyle/outputprogramparameter
prompt: Standard error
filenames: '"fasta.err"'
name: stderr
type: !mobyle/formattedtype
data_terms: ['EDAM_data:2048']
references:
- {doi: null, label: 'Pearson, W. R. (1999) Flexible sequence similarity searching
with the FASTA3 program package. Methods in Molecular Biology', url: null}
- {doi: null, label: 'W. R. Pearson and D. J. Lipman (1988), Improved Tools for Biological
Sequence Analysis, PNAS 85:2444-2448', url: null}
- {doi: null, label: 'W. R. Pearson (1998) Empirical statistical estimates for sequence
similarity searches. In J. Mol. Biol. 276:71-84', url: null}
- {doi: null, label: 'Pearson, W. R. (1996) Effective protein sequence comparison.
In Meth. Enz., R. F. Doolittle, ed. (San Diego: Academic Press) 266:227-258',
url: null}
homepage_links: ['http://fasta.bioch.virginia.edu/fasta_www2/fasta_list2.shtml']
env: {}
source_links: ['http://faculty.virginia.edu/wrpearson/fasta/']