No splice site and ancestral allele annotations

[Filago4]

Hello,

I used Loftee with the standard VEP pipeline (V. 105)

Here you can find the relevant part of my command:
--plugin LoF,loftee_path:/home/myname/documents/vep_plugins/loftee,human_ancestor_fa:/home/myname/documents/vep_plugins/loftee/human_ancestor.fa.gz,conservation_file:/home/myname/documents/vep_plugins/loftee/phylocsf_gerp.sql,gerp_file:/home/myname/documents/vep_plugins/loftee/GERP_scores.final.sorted.txt.gz,fast_length_calculation:slow

When I run the code, it seems to work, but:
A) this error appears several times:
"Use of uninitialized value $faidx in split at /home/myname/.vep/Plugins/LoF.pm line 549."

B) My results dont show a single hit for "human_ancestor_fa" or the splice site predictions like e.g. "NON_DONOR_DISRUPTING", but a lot for "END_TRUNC" and also some for the "utr"-predictions

This seems wrong to me...any suggestions?

Furthermore, I have some more small questions:
C) is this annotation in my code correct:
fast_length_calculation:slow or do I have to set it to "0" or "1"?

D) Is the "GERP_scores.final.sorted.txt.gz"-file necessary?

Many thanks in advance! :)