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Reproducibility of human tonsil data analysis #4

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Irisapo opened this issue Sep 18, 2022 · 0 comments
Open

Reproducibility of human tonsil data analysis #4

Irisapo opened this issue Sep 18, 2022 · 0 comments

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@Irisapo
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Irisapo commented Sep 18, 2022

Hi,

Can you guys help me in reproducing the clustering and gene score results on the human tonsil data?
There might be something I did wrong, as I am only able to get 2 clusters, and half of the genes predicted non-zero scores with the parameter settings you guys have in the script/paper.
Here is what I did:

I filtered out 12 spots as you guys did, 
I then used the 2488 remaining spots to do dimensionality reduction (addIterativeLSI) and clustering (addClusters). 
The gene scores were then calculated using addImputeWeights. 
All the parameters I used are the same from the manuscript/your script. 

As I am noticing that the script you guys provided here are specific for mouse datasets. I wondering whether I have missed anything.

Thank you for your help in advance!
Shiyi

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