Job submission error for particle stacks

[mcianfrocco]

Hey Mike,

I told you the other day that your AWS wrapper doesn't like data that is not fully processed in Relion (or at least it doesn't like how I'm trying to use it).

I have been processing the negative stain data from 17apr30b and run into this issue. For my workflow, I made a phase-flipped particle stack in IMAGIC format using Appion. I preprocessed this using relion_preprocess and then did 2D classification on one of our machines. When I selected the best classes and tried to run 3D classification, I got this error. I can't remember which data set I had a similar problem with when trying to do 2D classification through AWS, but I'm pretty certain it's the same error as this one.

Hope this helps!

Rick

Traceback (most recent call last):

  File "/home/michaelc/Scripts/AWS//relion//qsub_aws", line 3612, in

    relion_refine_mpi(project)

  File "/home/michaelc/Scripts/AWS//relion//qsub_aws", line 897, in relion_refine_mpi

    otherPartDir,otherPartRclone,error=checkPartLocation(starfilename,particledir)

  File "/home/michaelc/Scripts/AWS//relion//qsub_aws", line 508, in checkPartLocation

    if microcolnum == 0:

UnboundLocalError: local variable 'microcolnum' referenced before assignment