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parameter usage for RNA sequencing samples #15

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mahwish2 opened this issue Aug 4, 2021 · 2 comments
Open

parameter usage for RNA sequencing samples #15

mahwish2 opened this issue Aug 4, 2021 · 2 comments

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@mahwish2
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mahwish2 commented Aug 4, 2021

Hi, I have multiple bam files (test1.bam test2.bam test3.bam) and the genome fasta and annotation gff.
I want to visualize it with jigv. Could you please post the command for this.

Thanks

@mahwish2
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mahwish2 commented Aug 5, 2021

When I simply try to use the following:

jigv
--fasta xxx.fa
--sites xxx_full.bed
/path/jigv/*.bam > regions.html

I get the following error:
Error: unhandled exception: /home/brentp/src/jigv/jigv.nim(376, 12) found.len == 1 [jigv] found {} sample names (SM read-group tags in bam header), expected exactly one. [AssertionError]

Also to mention, I am not sure about --sites parameter, since in my case I am not interested in particular region.

Thanks

@brentp
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brentp commented Aug 10, 2021

hi, jigv expects your bam files to have proper read-groups, it seems that's not the case.
those allow jigv to get the sample name.

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