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FitHiChIP requests different input than specified in documentation #104

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roniweiss12 opened this issue Feb 4, 2024 · 1 comment
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@roniweiss12
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Hello,
when running FitHiChIP via docker with the following command:
bash ./FitHiChIP_Docker.sh -C /path/to/fithichip_config
I eventually receive this:
================ Verifying input configuration parameters =================
There are three ways to provide FitHiChIP input:

  1. provide valid pairs file (from HiC pro pipeline),
  2. Provide both bin interval and matrix files (from HiC pro pipeline), or
  3. provide a set of locus pairs along with their contact counts (7 columns).
    But user did not provide any of these input configurations. - exit !!

even though the documentation says that an .mcool file can be used as input.
This is how my config file looks:

COOL="/path/to/sertoli.mcool"
PeakFile="/path/to/file.bed"
OutDir="/path/to/fithichip_results"
ChrSizeFile="/path/to/mm10.chrom.sizes"
BINSIZE=5000
QVALUE=0.01
UseP2PBackgrnd=0
MergeInt=1
PREFIX="fitHiChIP_5k"
OverWrite=1

What am I missing here?
Thanks!

@ay-lab
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ay-lab commented Mar 8, 2024

Hi @roniweiss12
Sorry for the late reply. Please use FitHiChIP_HiCPro.sh and the conventional installation. The Docker and Singularity versions are outdated.

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