Skip to content

Commit

Permalink
Version 3.0.0
Browse files Browse the repository at this point in the history
  • Loading branch information
armintoepfer committed Aug 7, 2018
1 parent 1f8ff79 commit d2107e4
Showing 1 changed file with 7 additions and 3 deletions.
10 changes: 7 additions & 3 deletions README.md
Original file line number Diff line number Diff line change
Expand Up @@ -40,6 +40,10 @@ not via mail to developers.

Binaries require **SSE4.1 CPU support**; CPUs after 2008 (Penryn) include it.

## Changelog

* 3.0.0: Initial release, included in SMRT Link 6.0.0

## SMRTbell designs

PacBio supports three different SMRTbell designs for IsoSeq libraries.
Expand Down Expand Up @@ -171,7 +175,7 @@ Example invocation:
- *ccs*: Get it from the official [SMRT Link](https://www.pacb.com/support/software-downloads/) or compile your own from [unanimity](https://github.com/PacificBiosciences/unanimity)
- *lima*: Pre-compiled binary from [barcoding](https://github.com/pacificbiosciences/barcoding)
- *isoseq3*: Pre-compiled binaries from [releases](https://github.com/PacificBiosciences/IsoSeq3/releases)

Add the directory containing the binaries to `PATH`:

```
Expand Down Expand Up @@ -242,14 +246,14 @@ subreads to polished isoforms; timings are system dependent:
user 2832m8.382s
$ ls polished*
polished.bam polished.bam.pbi polished.hq.fasta.gz polished.hq.fastq.gz polished.lq.fasta.gz polished.lq.fastq.gz polished.transcriptset.xml

If you have multiple cells, you should run `--split-bam` in the cluster step which will produce chunked cluster results. Each chunked cluster result can be run as a parallel polish job and merged at the end. The following example splits into 24 chunks. `sample.subreadset.xml` is the dataset containing all the input cells. The `isoseq3 polish` jobs can be run in parallel.

$ isoseq3 cluster demux.primer_5p--primer_3p.bam unpolished.bam --split-bam 24
$ isoseq3 polish unpolished.0.bam sample.subreadset.xml polished.0.bam
$ isoseq3 polish unpolished.1.bam sample.subreadset.xml polished.1.bam
$ ...


## FAQ
### Why IsoSeq3 and not the established IsoSeq1 or IsoSeq2?
Expand Down

0 comments on commit d2107e4

Please sign in to comment.