For detail of Optical System

[kip-bit]

Dear Author,
Thank you for your great work! Actually, I am new to computational imaging, There are a few things I don't understand：
1.In the simulation phase mask, why use a 25*25 pixel pupil and then multiply it by 71/25 at the the relative displacement?
2.I want to ask which plane the in-focus depth refers to from the object plane.
3.I would like to know how to calculate the focal length of a microscopic optical system, in order to calculate the spatial domain size of a simulated psf.
Please help me! Thank you very much！

[MJ2695]

I'm sorry for not getting back to you sooner.

1. The 25 is on the pupil plane, and the 71 is for the image plane. The numbers are calculated from the pixel size of the sensor, the magnification of the system, and Nyquist sampling rate, etc.
2. I'm not sure if I understood your question correctly. The in-focus depth is calculated using the focal length of the microscope objective. We assume that the object (I assume what you mean by object plane) will be within +- 100 um of the objective's best focus plane. Outside of this range we see a decrease in image quality.
3. This should be given by the microscope objective. We used one from Olympus with an effective focal length of 45 mm.

For more details, you can see the paper and the supplemental. Feel free to email us with any further questions.